Abstract Objective Gossypetin, isolated from Hibiscus sabdariffa L, has been shown to have various pharmacological effects including anti-inflammatory and antibacterial activity against various diseases. However, since the effect of gossypetin in oral cancer remains to be reported, we aimed to investigate the anticancer activity and mechanisms of gossypetin in oral squamous cell carcinoma (OSCC). Methodology The proliferation of OSCC cells was evaluated by cell viability and soft agar colony assays. The effects of gossypetin on the migration and invasion of OSCC cells was investigated by wound healing and transwell invasion assays, respectively. Apoptosis and cell cycle arrest were measured by flow cytometry. Moreover, the anticancer mechanism of gossypetin in OSCC cells was analyzed by western blotting. Results Gossypetin inhibited the proliferation, migration, and invasion of OSCC cells and induced apoptosis by upregulating the Bax/Bcl-2 ratio and cell cycle arrest at the G2/M phase. Furthermore, gossypetin regulated the activation of extracellular signal-regulated kinase and nuclear factor-kappa B. Conclusion Results showed that gossypetin inhibits the proliferation, migration, and invasion of OSCC cells and triggers apoptosis and cell cycle arrest in OSCC. Therefore, gossypetin has the potential for use as a chemopreventive agent in oral cancer.

Nanotubos de carbono de multi-paredes oxidados (ox-MWCNT) foram utilizados como condutores adicionais para preparar uma nova rede de cátodos para bateria de íons de lítio. A morfologia foi analisada pelo microscópio de varredura eletrônico, e partículas ativas de LiMn2O4 foram ligados ao ox-MWCNT com a formação da rede tridimensional de fiação. O transporte de elétrons e a atividade eletroquímica foram melhorados de forma eficaz. Testes galvanostáticos de carga-descarga do cátodo LiMn2O4/ox-MWCNT mostrou que a capacidade de descarga inicial são 119,4, 110,6, 105,5 e 91,4 mAh g-1 à taxa de 0,1, 0,5, 1 e 2 C, respectivamente, os quais são muito superiores aos de LiMn2O4/acetileno (AB), de mesmo conteúdo. A espectroscopia da impedância eletroquímica AC mostrou que a resistência da transferência de carga (Rct) do eletrodo composto reduziu de 34,32 Ω para LiMn2O4/ox-MWCNT, em comparação ao valor de 53,2 Ω para LiMn2O4/AB. De um modo geral, se verificou que uma rede condutora para facilitar a transferência de elétrons e fazer uma boa conexão das partícula ativas com o material da rede desempenham um papel importante para avaliar a capacidade e eficiência do ciclo.

Oxidized multi-walled carbon nanotubes (ox-MWCNT) were used as conducting addition to prepare a novel network composite cathode for lithium ion battery. The morphology was analyzed by scanning electron microscope, LiMn2O4 active particles were connected by ox-MWCNT with the formation of three-dimensional networking wiring. Electron transport and electrochemical activity were improved effectively. Galvanostatic charge-discharge tests of LiMn2O4/ox-MWCNT cathode showed that the initial discharge capacities are 119.4, 110.6, 105.5 and 91.4 mAh g-1 at the rate of 0.1, 0.5, 1 and 2 C, respectively, which were much higher than LiMn2O4/acetylene black (AB) at the same content. The electrochemical AC impedance spectroscopy showed that the charge transfer resistance (Rct) of the composite electrode reduced obviously contrasting to 34.32 Ω for LiMn2O4/ox-MWCNT and 53.2 Ω for LiMn2O4/AB. Overall, it is found that a conductive network to facilitate electron transfer and good connection of the active-material particle to the network were playing an important role to rate capability and cycle efficiency.

Este trabalho apresenta o projeto e a preparação bem sucedidos de um filme de alumina porosa anódica do tipo sanduíche (PAA/Al2O3/PAA). Utilizou-se o processo usual, em duas etapas, de anodização de ambos os lados de uma folha de alumínio. A estrutura do filme foi determinada por microscopia eletrônica de mapeamento e emissão de campo (SEM). Os resultados obtidos na microscopia SEM mostraram que as membranas PAA têm uma nano estrutura bem definida. Todos os nano buracos apresentaram-se de forma simétrica em ambos os lados da camada de barreira. O diâmetro médio do poro encontrado foi da ordem de 40 nm.

A sandwich porous anodic alumina (PAA/Al2O3/PAA) film was successfully designed and fabricated using the customary two-step anodization approach on both sides of an aluminum foil. The structure of the film was determined with field emission scanning electron microscopy (SEM). The SEM shows the PAA membranes has a well-defined nanostructure. All nanoholes stand symmetrically on both sides of the barrier layer. The average pore diameter reaches 40 nm.